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Universal DNA/RNA extract kit for extraction most microorganism including probiotics (50T)

Sale!

$89.00

Universal DNA/RNA extract kit for mammalian cells,  bacteria and fungal. It is very efficient, high-quality, and fasts.

Universal DNA/RNA extract kit for extraction of most microorganisms from the host with magnetic beads 50T

It is good for the DNA from probiotics too.

It is very easy to use. The procedure is short about 1 hour.

1, Prepare solid samples

a.     Solid sample: take about 60 mg (maximum for lysis buffer 300 µl) of tissue. Homogenize it with 130 µl PBS.

b.     Liquid samples directly use 130 µl.

If using a swab sample, 300 µl lysis buffer can be directly used to dissolve the samples.

Solid samples µl
Sample (PBS) 130
Lysis buffer 300
Proteinase K (10mg/ml Sigma) Not included in the kit 20
Magnetic beads (20mg/ml) 300
Isopropanol ( Not included in the kit) 450
Ethanol (70%)  Not included in the kit 500

2, Add 20 ul of proteinase K into the mixture above and incubate it at 56 ℃ for 60 min.

3, Add 300µl magnetic beads and mix well for 1 min. The binding buffer 450µl (100% Isopropanol) is added to the extraction mixture and rotated for 5 min at room temperature.

4, Setup the tube on the magnet and remove the supernatant.

5, Add 1 ml wash buffer (70% alcohol) and vortex for 10 seconds. Setup the tube on the magnet separation rack for 1-2 min and remove supernatant. Repeat the step 3 times.

6, Setup the tube on the magnet rack for 2 min and completely remove the wash buffer.

7, Add 100 µl elution buffer (autoclaved ddH2O, or TE buffer) and set up on heat block for 3 min at 65 °C.

8, Collect the eluted solution into a new autoclaved tube for future use.

Note: 1, the Sample volume is adjustable. As long as keep a ratio. Sample: Lysis buffer:PK:Beads: Binding Buffer; 130:300:20:300:450.

2, If the test will use RNA of the sample, make sure to prevent RNase contamination in all solutions.

Liquid samples

New ratio 400µl reaction 800µl reaction
Lysis buffer 150 300
Magnetic beads (20mg/ml) 100 200
Isopropanol ( Not included in the kit) 150 300
wash buffer Ethanol (70%) Not included in the kit 750 1500

1, Directly dissolve the swab sample into 300 µl lysis buffer and 20 ul proteinase K in a 1-ml Eppendorf tube.  Incubate for 30 min at 65 °C.

Note: if the liquid sample needs about 5-10 min.

3, Add 200µl magnetic beads and mix well for 1 min. The binding buffer 300µl (100% Isopropanol) is added to the extraction mixture and rotated for 5 min at room temperature.

4, Setup the tube on the magnet and remove the supernatant.

5, Add 1 ml wash buffer (70% alcohol) and vortex for 10 seconds. Setup the tube on the magnet separation rack for 1-2 min and remove supernatant. Repeat the step 3 times.

6, Setup the tube on the magnet rack for 2 min and completely remove the wash buffer.

7, Add 100 µl elution buffer (autoclaved ddH2O, or TE buffer) and set up on heat block for 3 min at 65 °C.

8, Collect the eluted solution into a new autoclaved tube for future use.

Note: 1, the Sample volume is adjustable. As long as keep a ratio. Sample: Lysis buffer:PK:Beads: Binding Buffer; 130:300:20:300:450.

2, If the test will use RNA of the sample, make sure to prevent RNase contamination in all solutions.

It also is available in eBay Universal DNA/RNA Kit. If you want to extract DNA/RNA from blood you can click here. Whole blood DNA/RNA extraction.

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