DNA purification Making Lentiviral and AAV particles
If you want to get high pure DNA , you are in the right place. We provide a fast and quality service for genomic DNA and plasmid DNA with magnetic nanoparticles.
DNA extraction
We provide DNA extraction and purification in high quality. We can extract DNA from animal or human tissues, cells, blood, plasma, saliva, urine, stool and plasmid from bacterial.
Conjugation
We have magnetic nanoparticle with multiple groups, such as -NH2, -COOH and siO2. We can help costumers to conjugate the protein, antibody and DNA on the nanoparticles.
Cell transfection
Transfecting DNA to cells is an important for scientist. However, transfection efficiency from regular lipid is limited and have toxicity to cells. Our Trans-GN provides a high transfection efficient for the most of cells.
We are expertise on extraction DNA
DNA EXTRACTION
We have specific MNP-SiO2 to isolate DNA from different sources and had high quality of genomic and plasmid DNA. The ratio of A160/A280=1.8-1.9. The DNA can be used for NGS, qPCR and other down stream utilities.
MAGNETIC NANOPARTICLE COATING
We have MNP with two activating groups, -NH2 and -COOH. The group provide chance to conjugate to protein and DNA for biomedical use. We can help customers' to conjugate protein, antibody and DNA on the MNP.
LENTIVIRAL PACKAGING AND PURIFICATION
Chimerical antigen receptor (CAR) T cell (CAR-T) has been used to treat cancers. A critical step is a consistent expression CAR in T cells. Therefore purity of lentivial vector is very important. We help costumers' to purify the vectors and particles to guarantee successful cotransfection and transduction.
CELL TRANSFECTION
Using a new transfection reagent MNP-PEI, we can transfect plasmid into different cell line and primary cells. It has less toxicity than lipids.
AAV PACKAGING AND PURIFICATION
We can package Adeno-associate virus and purify. We can purify packaged AAV using magnetic nanoparticles. and ion exchange column. The purity is better than one purified by ultra-centrifuge with cscl.
DETECT GENOMIC DNA FROM STOOL OR SALIVA
Collateral cancer expresses genes in early stage. GI Doctor suggests to do a test after 50 years old. It will help earlier or predict if people will suffer a collateral cancer. Currently, clinical use endoscopy to do the examination. However, it take long time and patients suffer hungry and diarrhea for about a week. The gene test will replace the endoscopy. (this test is not available now)
