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Saliva DNA/RNA extraction kit with magnetic nanoparticles (100T)

Sale!

$259.00

Saliva DNA/RNA extraction kit with magnetic nanoparticles. It is easy, fast, high yield, and high quality.

Saliva DNA/RNA extraction kit with magnetic nanoparticles is an economic, easy, efficient, and high recovery yield. The kit has almost 100% yield and high quality.

Reagents Volume ml
Protection buffer 100
Lysis buffer 20
Wash buffer I 30
Wash buffer II 30
Elution buffer 5
Magnetic Beads 2
Isopropanol 15
β-Mercacptoethanol 1

1, collect 1-2 ml saliva add half of the protection buffer, and centrifuge it at 2500 RPM for 1 minute. Discard supernatant. About 100 µl residue pallet for next extraction.

2, Add 100µl lysis buffer into the residue pallet and mix well. Add 20 µl magnetic beads (25mg/ml) and 128 µl isopropanol into the mixture. Mix them very well at room temperature.

3, Set the tube on the magnet for 20 seconds. Remove the supernatant.

4, Add 300µl wash buffer I and vortex it. Set it up on the magnet rack and remove the supernatant.

5, Add 300 µl wash buffer II and vortex it. Set it up on the magnet rack and remove the supernatant.

6, Add 200 µl 0 ℃ cold water gently and quickly wash it. Set it on the magnet rack and rapidly remove the supernatant.

7, Add 50 µl elution buffer and vortex it well. Set it at 50 ℃ for 5 min. Set it on the magnetic rack and collect about 50µl supernatant. Save it in -80 ℃ for next use.

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